Journal of Developmental Medicine(Electronic Version)  2013, Vol. 1 Issue (1): 7-10 DOI: |
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| The effect of in Vitro maturatiOn on the expression and the regulatiOn of maternal imprinted genes in the testis of male offspring |
| WANG Li-ya, WANG Ning, LE Fang, et al
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| Center of Reproductive Medicine,women's Hospital．School of Medicine，Zhejiang UniverSity,Hangzhou 310006，China
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Abstract 【Abstract】objectives The aim of this research was to study the effect of in vitro maturation on the expression and the regulation of matemal imprinted genes in the testis of male offspring．methods Institute for clinical systems impmvement(ICSI)and in vitro maturation(IVM)conceived mice were established，and Quantitative real time RT—PCR and Pyrosequencing were used to detect the expressions and methylation levels of 6 matemalimprinted genes in their testes．Results Compared with ICSI mice，the increased expressions and decreased methylation levels were shown in IVM mice．Conclusion IVM may innuence the expressions of matemal imprinted genes in testis by regulating epigenetic modification．
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Received: 18 November 2012
Published: 06 September 2019
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