Journal of Developmental Medicine(Electronic Version)  2021, Vol. 9 Issue (2): 114-121 DOI: 10.3969/j.issn.2095-5340.2020.02.006 |
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| Specific expression of intestinal flora in pregnant women with gestational diabetes mellitus after assisted reproductive technology |
| Wang Fang, Zheng Jianlan, Wang Wenyan, et al
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1. Department of Obstetrics, ChenggongHospital Affiliated to Xiamen University, Army Group 73th Military Hospital, Fujian, Xiamen 361003, China; 2. Department of Obstetrics, Binhu Hospital, Anhui, Hefei 230601, China
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Abstract 【Abstract】 Objective By detecting and comparing the intestinal flora of pregnant women with different mode of conception and whether they were complicated with gestational diabetes mellitus (GDM), to find the specific expression of intestinal flora in GDM pregnant women after assisted reproductive technology (ART), and analyze its mechanism, so as to provide theoretical basis for the prevention and intervention in the future and the promotion of maternal and infant health after ART. Methods A total of 60 pregnant women whowere hospitalized and gave birth after regular prenatal check-up in the Department of Obstetrics, Chenggong Hospital Affiliated to Xiamen University (Army Group 73th Military Hospital) from February to April 2016 were recruited. According to different mode of conception, they were divided into four groups: ART complicated with GDM (n=18), ART no complicated with GDM (n=14), spontaneous pregnancy complicated with GDM (n=17) and spontaneous pregnancy no complicated with GDM (n=11). General clinical data including height, weight, age, gestational age, place of residence, pregnancy complications, diet in the last three days, as well as delivery mode and delivery outcome were recorded. 5-10 grams feces of these pregnant women were collected respectively at the time of admission or 24 hours before delivery to extract the intestinal flora DNA of the samples. Meanwhile, based on the 16S rRNA gene sequencing technology, the distribution of intestinal flora in the samples was analyzed for the subsequent quantitative study of the flora and the analysis of metabolites. Results The four groups of samples were mainly composed of Firmicutes and Bacteroidetes. No matter what kind of pregnancy mode, the Bifidobacteria in GDM group was less than that in other groups. The abundance of Firmicutes in the intestinal flora of ART pregnancy women was downregulated,
and the abundance of Proteus increased (P<0.05). The diversity of Alpha in intestinal flora of ART
pregnancy women decreased (P<0.05). The abundance and diversity of intestinal flora in ART complicated
with GDM group were reduced, and the composition proportion of intestinal flora was out of balance, and
Klebsiella pneumoniae with higher abundance was found. The Alpha diversity of ART complicated with
GDM group and ART no complicated with GDM group were relatively consistent, while the Alpha diversity
between spontaneous pregnancy complicated with GDM group and spontaneous pregnancy no complicated with GDM group were significantly different. Conclusions Bifidobacteria supplementation may help to prevent or treat GDM. The proportion of intestinal flora composition and structure of ART complicated with GDM group is imbalance. The use of large doses of hormone during ART will lead to the imbalance of intestinal flora of pregnant women. The change of intestinal flora caused by ART is far greater than the effect of diabetes on intestinal flora.
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Received: 14 May 2020
Published: 01 April 2021
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