Journal of Developmental Medicine(Electronic Version)  2021, Vol. 9 Issue (3): 206-213 DOI: 10.3969/j.issn.2095-5340.2021.03.008 |
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| Study on the mechanism of Bu-Shen-Huo-Xue Recipe in the treatment of recurrent implantation failure based on network pharmacology and bioinformatics |
| Xia Lixian,Ye Qian, Zou Li, et al
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1. Fuwai Hospital, National Cardiovascular Center, Chinese Academy of Medical Sciences, Health Department, Beijing 100037, China; 2. Jiangxi University of Traditional Chinese Medicine, Graduate School, Jiangxi,Nanchang 330004, China; 3. Jiangxi Provincial Maternal and Child Health Hospital, TCM department, Jiangxi Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Female Reproduction, Jiangxi,Nanchang 330006, China
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Abstract 【Abstract】 Objective Network pharmacology and bioinformatics were used to analyze the molecularmechanism of Bu-Shen-Huo-Xue Recipe (BSHXR) in the treatment of recurrent implantation failure (RIF). Methods The database TCMSP and BATMAN-TCM were retrieved, and the active components and targets of BSHXR were obtained. Differential genes were obtained by using GEO database. The known target genes related to RIF were obtained by searching GeneCards database. The action targets of BSHXR and the two database genes were intergrated for common genes. Meanwhile, Cytoscape 3.7.2 software was used to construct the common gene network map, and the network diagram was constructed based on herbcomponent-target -pathway. Finally, the DAVID database was used for GO and KEGG analysis of common genes. Results A total of 277 targets were retrieved from BSHXR. GEO2R obtained 390 significantly different genes. from GEO2R. There are 3 454 RIF-related targets. The mapping of drug target and disease target resulted in 11 common genes. GO analysis showed that the intersection target proteins were mainly involved in multi-cellular homeostasis, endothelial cell migration, lipid biosynthesis, positive regulation of hydrolase activity, epithelial cell migration, smooth muscle contraction, tissue migration and other biological processes; cell composition of fossa, membrane raft, membrane microdomain, membrane region, plasma membrane raft, etc; molecular functions such as oxidoreductase activity, heme binding, tetrapyrrole binding, cofactor binding, protein homodimerization activity, etc. KEGG signaling pathway was mainly enriched in cancer pathway, chemical carcinogenesis, drug metabolism, calcium signaling pathway, etc. Quercetin, kaundol and luteolin were the main active components of BSHXF, and prostaglandin-endoperoxide synthase 2 (PTGS2), estrogen receptor 1 (ESR1), cytochrome P450 3A4 (CYP3A4), 5-hydroxytryptamine receptor 2A (HTR2A) were the main targets of action. Molecular docking showed that quercetin, kaolferol and luteolin could stably bind to PTGS2, ESR1, CYP3A4 and HTR2A. Conclusions A variety of active compounds in BSHXF, such as quercetin, kaanol and luteolin, may play a role in the treatment of RIF by acting on multiple key targets such as PTGS2, ESR1, CYP3A4 and HTR2A to regulate multiple signaling pathways such as cancer pathway, chemical carcinogenesis, drug metabolism and calcium signaling pathway.
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Received: 18 April 2021
Published: 27 May 2021
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