Journal of Developmental Medicine(Electronic Version)  2023, Vol. 11 Issue (4): 249-255 DOI: 10.3969/j.issn.2095-5340.2023.04.002 |
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| Short term of anti-inflammatory efficacy observation induced by Vicryl suture coated with silver nanoparticles in mice dorsal intradermal running suture models |
| Liu Xuelai, He Feng, Song Yanbiao, et al
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| 1.Department ofSurgery, Capital Institute of Pediatrics Affiliated Children Hospital, Beijing 100020, China; 2. Department of Biochemistry and Immunology, Capital Institute of Pediatrics, Beijing 100020, China; 3. Central Laboratory, the Second Hospital of Hebei Medical University, Hebei, Shijiazhuang 050061, China; 4. Diabetic FootDiagnosis and Treatment Center, Jilin Province People's Hospital, Jilin, Changchun 130021, China) |
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Abstract 【Abstract】 Objective To investigate the expression of inflammatory cells infiltration and inflammatory
proteins around silver nanoparticles coated Vicryl suture in mice dorsal intradermal running suture models,
and to analyze the anti-inflammatory effect. Method The 4-0 Vicryl suture were coated with silver
nanoparticles by chemical coating technique. Eighteen Balb/C mice at 10-12 weeks were randomly divided
into 3 groups (with 6 mice in each group). The back hair of the mice was shaved and a 15 mm longitudinal
full-layer incision was made. Continuous intradermal suture was performed using 4-0 common Vicryl suture,antibiotic Vicryl suture, and silver nanoparticles coated Vicryl suture, respectively. The whole skin tissue wascollected with longitudinal incision as the center at 5 days after surgery. Immunohistochemical staining wasused to compare the expression levels of neutrophils, macrophages, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) around the channels. The mean optical density (MOD) was calculated. Mediated dUTPbiotin nick end labeling (TUNEL) staining was used to investigate the apoptosis of cells around the channels. Masson staining was used to investigate the collagen deposition around the channels. One-way analysis of variance and student't t-test were used for statistical analysis. Result The immunohistochemistry staining indicated neutrophil MOD in common Vicryl suture group, antibiotic Vicryl suture group and silver nanoparticles coated Vicryl suture groups was 0.523±0.137, 0.412±0.122, 0.229±0.079, respectively; macrophage MOD in above three groups was 1.125±0.126, 0.585±0.142, 0.209±0.078, respectively; IL-6 MOD in above three groups was 1.137±0.168, 0.652±0.131, 0.227±0.086, respectively; TNF-α MOD in above three groups was 1.219±0.124, 0.573±0.109, 0.179±0.045, respectively; the infiltration of inflammatory cells, expression of IL-6 and TNF-α was most obvious in common Vicryl suture group and least obvious in silver nanoparticles coated Vicryl suture group; the differences among the three groups were statistically significant (all P<0.001). TUNEL staining showed that MOD of apoptosis cells in common Vicryl suture group, antibiotic Vicryles suture group and silver nanoparticles coated Vicryl suture groups was 0.167±0.035, 0.478±0.151, 0.125±0.035, respectively; most apoptosis cells could be seen in antibiotic Vicryl suture group, and least apoptosis cells were found in silver nanoparticles coated Vicryl suture groups; the differences among the three groups were statistically significant (all P<0.001). Masson staining showed that most collagen deposit could be seen in silver nanoparticles coated Vicryl suture groups, but no collagen deposit could be found in antibiotic Vicryl suture group. Conclusion Compared with either common Vicryl suture or antibiotic Vicryl suture, the silver nanoparticles coated Vicryl suture presents better short term of antiinflammatory efficacy and weak cytotoxicity in mice dorsal intradermal running suture models. Although this,the potential clinical application value needs to be further evaluated.
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Received: 18 April 2023
Published: 31 July 2023
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