发育医学电子杂志 Vol. 1 205-209
临床遗传
论著
长链非编码RNA PVT1对MDA-MB-231细胞 增殖和侵袭能力的影响
王少冰 王芳
第二军医大学基础医学部医学遗传学教研室
Influence of lncRNA-PVT1 on proliferation and invasion of MDA-MB-231 cells
WANG Shao-bing,WANG Fang
Basic Medical Department of the Second Military Medical University Medical Genetics Teaching and Research Section
摘要 【摘要】 目的 探讨长链非编码RNA PVT1（lncRNA-PVT1）对于乳腺癌细胞系MDA-MB-231 增 殖和侵袭的影响。方法 构建pcDNA3.1-PVT1 表达载体，并将其转染入MDA-MB-231 细胞，用实时 定量PCR 方法检测转染细胞中lncRNA-PVT1 的表达，用Edu 标记技术检测转染细胞的增殖能力，用 ranswell 实验检测转染细胞的侵袭能力。结果 构建成功的pcDNA3.1-PVT1 重组质粒转染入MDAMB- 231 细胞后lncRNA-PVT1 表达增加；转染细胞的增殖能力增强；转染细胞的侵袭能力增强。结论 lncRNA-PVT1 能增强MDA-MB-231 细胞的增殖和侵袭能力。
关键词:
长链非编码RNA ')" href="#">长链非编码RNA
PVT1
乳腺癌
Abstract: 【Abstract】 Objectives To study the effect of ectopic expression of long Noncoding RNA PVT1 in MDAMB-231 cells. Methods The pcDNA3.1-PVT1 plasmids were transfected into MDA-MB-231 cells and the expression of lncRNA-PVT1 was detected by Real time PCR. The proliferation ability of transfected cells was evaluated by Edu based assay. The invasion ability of transfected cells was evaluated by Transwell experiment. Results The expression of lncRNA-PVT1 was increased when the recombinant plasmids were transfected into MDA-MB-231 cells. The proliferation ability of transfected cells was improved and so was the invasion ability. Conclusion Long noncoding RNA PVT1 can improve the proliferation and invasion of MDA-MB-231 cells.
Key words:
long noncoding RNA')" href="#">long noncoding RNA
PVT1
Breast cancer
收稿日期: 2013-10-01
出版日期: 2013-10-30
发布日期: 2019-07-22
期的出版日期: 2013-10-30
基金资助: 国家自然科学基金（81372240，81171937）
通讯作者:
王芳
E-mail: wfsjz@163.com
引用本文:
王少冰 王芳. 长链非编码RNA PVT1对MDA-MB-231细胞 增殖和侵袭能力的影响 [J]. 发育医学电子杂志, 2013, 1(4): 205-209.
Influence of lncRNA-PVT1 on proliferation and invasion of MDA-MB-231 cells . Journal of Developmental Medicine(Electronic Version), 2013, 1(4): 205-209.
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