辅助生殖技术,妊娠期糖尿病,肠道菌群,菌群多样性," /> 辅助生殖技术,妊娠期糖尿病,肠道菌群,菌群多样性,"/> Assisted reproductive technology, Gestational diabetes mellitus, ,Intestinal flora,Flora diversity
,"/> <span style="font-size:14px;line-height:2;">辅助生殖技术与妊娠期糖尿病孕产妇 </span><span style="font-size:14px;line-height:2;">肠道菌群的特异表达</span>
Please wait a minute...
欢迎访问发育医学电子杂志,今天是
发育医学电子杂志  2021, Vol. 9 Issue (2): 114-121    DOI: 10.3969/j.issn.2095-5340.2020.02.006
  围产医学   论著 |产科 |
辅助生殖技术与妊娠期糖尿病孕产妇 肠道菌群的特异表达
王方 郑剑兰 汪文雁 彭芳林
1. 厦门大学附属成功医院 陆军第73 集团军医院 产科,福建 厦门 361003;2. 合肥市滨湖医院 产科,安徽 合肥 230601
Specific expression of intestinal flora in pregnant women with gestational diabetes mellitus after assisted reproductive technology
Wang Fang, Zheng Jianlan, Wang Wenyan, et al
1. Department of Obstetrics, ChenggongHospital Affiliated to Xiamen University, Army Group 73th Military Hospital, Fujian, Xiamen 361003, China; 2. Department of Obstetrics, Binhu Hospital, Anhui, Hefei 230601, China
下载:  PDF (1630KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 【摘要】 目的  通过检测和比较不同受孕方式及是否并发妊娠期糖尿病(gestational diabetes mellitus,
GDM)孕产妇的肠道菌群,明确辅助生殖技术(assisted reproductive technology,ART)及GDM 孕产妇肠道菌群的特异性表达,分析其机制,为今后预防干预、促进ART 妊娠母婴健康提供理论基础。 方法 选取
2016 年2 月至4 月在厦门大学附属成功医院(陆军第73 集团军医院)产科定期产检后住院分娩的足月
妊娠、近3 个月内未使用过抗生素并无肠道疾病的孕产妇,按ART 和自然妊娠两种不同受孕方式及是
否并发GDM 纳入60 例,分为4 组,分别为ART 并发GDM 组18 例、ART 无GDM 组14 例、自然妊娠
并发GDM 组17 例和自然妊娠无GDM 组11 例,记录孕妇的身高、体重、年龄、孕周、居住地、妊娠合并
症并发症、近3 d 饮食情况,以及分娩方式、分娩结局等临床资料;收集孕产妇入院或分娩之前24 h 的粪
便5 ~ 10 g;提取肠道菌群DNA;同时基于16S rRNA 基因测序技术,分析样本中肠道菌群的分布情况;
用于后续的菌群量化研究及代谢物分析。 结果  4 组样本的肠道菌群主要由厚壁菌门和拟杆菌门组
成;不论何种受孕方式,GDM 孕产妇的双歧杆菌均比其他孕产妇减少;ART 孕产妇肠道菌群的厚壁菌
门丰度降低,变形菌门所属菌群丰度增加(P<0.05),肠道菌群中的Alpha 多样性降低(P<0.05);ART 并发GDM 组肠道菌群的丰度、多样性降低,肠道菌群的组成比例失衡,且出现较高丰度的条件致病菌克雷伯
氏菌;ART 并发GDM 组和ART 无GDM 组的Alpha 多样性较为一致,而自然妊娠并发GDM 组和自
然妊娠无GDM 组之间的Alpha 多样性则存在明显差异。 结论 补充双歧杆菌可能有助于预防或者治
疗GDM ;ART 并发GDM 组的肠道菌群组成结构比例失调;ART 大剂量激素的使用会导致孕产妇的
肠道菌群失衡;ART 导致的肠道菌群变化远大于GDM 对肠道菌群的作用。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
关键词:  辅助生殖技术')" href="#">辅助生殖技术  妊娠期糖尿病  肠道菌群  菌群多样性    
Abstract: 【Abstract】 Objective By detecting and comparing the intestinal flora of pregnant women with different mode of conception and whether they were complicated with gestational diabetes mellitus (GDM), to find the specific expression of intestinal flora in GDM pregnant women after assisted reproductive technology (ART), and analyze its mechanism, so as to provide theoretical basis for the prevention and intervention in the future and the promotion of maternal and infant health after ART. Methods A total of 60 pregnant women whowere hospitalized and gave birth after regular prenatal check-up in the Department of Obstetrics, Chenggong Hospital Affiliated to Xiamen University (Army Group 73th Military Hospital) from February to April 2016 were recruited. According to different mode of conception, they were divided into four groups: ART complicated with GDM (n=18), ART no complicated with GDM (n=14), spontaneous pregnancy complicated with GDM (n=17) and spontaneous pregnancy no complicated with GDM (n=11). General clinical data including height, weight, age, gestational age, place of residence, pregnancy complications, diet in the last three days, as well as delivery mode and delivery outcome were recorded. 5-10 grams feces of these pregnant women were collected respectively at the time of admission or 24 hours before delivery to extract the intestinal flora DNA of the samples. Meanwhile, based on the 16S rRNA gene sequencing technology, the distribution of intestinal flora in the samples was analyzed for the subsequent quantitative study of the flora and the analysis of metabolites. Results The four groups of samples were mainly composed of Firmicutes and Bacteroidetes. No matter what kind of pregnancy mode, the Bifidobacteria in GDM group was less than that in other groups. The abundance of Firmicutes in the intestinal flora of ART pregnancy women was downregulated,
and the abundance of Proteus increased (P<0.05). The diversity of Alpha in intestinal flora of ART
pregnancy women decreased (P<0.05). The abundance and diversity of intestinal flora in ART complicated
with GDM group were reduced, and the composition proportion of intestinal flora was out of balance, and
Klebsiella pneumoniae with higher abundance was found. The Alpha diversity of ART complicated with
GDM group and ART no complicated with GDM group were relatively consistent, while the Alpha diversity
between spontaneous pregnancy complicated with GDM group and spontaneous pregnancy no complicated with GDM group were significantly different. Conclusions Bifidobacteria supplementation may help to prevent or treat GDM. The proportion of intestinal flora composition and structure of ART complicated with GDM group is imbalance. The use of large doses of hormone during ART will lead to the imbalance of intestinal flora of pregnant women. The change of intestinal flora caused by ART is far greater than the effect of diabetes on intestinal flora.

Key words:  Assisted reproductive technology')" href="#">Assisted reproductive technology    Gestational diabetes mellitus         Intestinal flora    diversity
')" href="#">Flora diversity
收稿日期:  2020-05-14                     发布日期:  2021-04-01     
基金资助: 厦门市卫生健康委科研项目(3502Z20184066)
通讯作者:  郑剑兰    E-mail:  sunnyzdoc@163.com
引用本文:    
王方 郑剑兰 汪文雁 彭芳林. 辅助生殖技术与妊娠期糖尿病孕产妇 肠道菌群的特异表达[J]. 发育医学电子杂志, 2021, 9(2): 114-121.
Wang Fang, Zheng Jianlan, Wang Wenyan, et al. Specific expression of intestinal flora in pregnant women with gestational diabetes mellitus after assisted reproductive technology. Journal of Developmental Medicine(Electronic Version), 2021, 9(2): 114-121.
链接本文:  
http://www.fyyxzz.com/CN/10.3969/j.issn.2095-5340.2020.02.006  或          http://www.fyyxzz.com/CN/Y2021/V9/I2/114
[1] 古灼和 陈赞雄 董光辉 曾晓雯 谢彦奇 李青青. 儿童体重指数与肠道菌群多样性的关联性研究[J]. 发育医学电子杂志, 2021, 9(2): 103-108.
[2] 梁杨 么大伟. 妊娠期糖尿病孕妇脂联素、趋化素、超敏C反应蛋白及胰岛素抵抗的表达及意义[J]. 发育医学电子杂志, 2019, 7(4): 282-286.
[3] 何素健 刘喜红 杨波 罗红凤 黎常红 肖妮蓉 徐明枝. 妊娠期糖尿病产妇母乳n-3多不饱和脂肪酸含量对婴儿早期生长发育的影响[J]. 发育医学电子杂志, 2019, 7(4): 274-281.
[4] 刘晓峰  金玉  齐宸  管滔  郭艳杰  关怀. 孕前超重对母婴肠道菌群的影响[J]. 发育医学电子杂志, 2019, 7(2): 126-129.
[5] 曹丹丹 马灌楠 董梦醒 等. 肠道菌群与儿童营养不良关系的研究进展[J]. 发育医学电子杂志, 2017, 5(1): 45-49.
[6] 李佳 侯新琳. 应用振幅整合脑电图评价妊娠期糖尿病对新生儿脑发育的影响[J]. 发育医学电子杂志, 2016, 4(4): 218-222.
[7] 王心 尚丽新. 妊娠中期糖尿病患者血清胱抑素C 水平与胰岛素抵抗关系的临床研究[J]. 发育医学电子杂志, 2015, 3(1): 39-41,49.
[1] Society of Neonatologist, Chinese Medical Doctor Association. Consensus recommendations on the prevention and early management of respiratory distress syndrome in preterm infants[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 129 -131 .
[2] Professional Committee of Respiratory, Society of Neonatologist, Chinese Medical Doctor Association. Clinical application recommendations for heated humidified high flow nasal cannula[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 132 -135 .
[3] LIU Shu-hua, SHEN Yue-bo, LIU Cui-qing, MA Li. The efficacy of pulmonary surfactant for pulmonary function in premature tension pneumothorax[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 146 -151 .
[4] GAO Xiao-hui, MAO Jian. Clinical features of non-oliguric hyperkalemia in extremely low birth weight infants[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 152 -158 .
[5] XIA Yao-fang, YANG Juan , TIAN Bao-li, et al. Value of amplitude-integrated electroencephalography in monitoring acute period of neonatal bilirubin encephalopathy and prognostic assessment[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 159 -163 .
[6] WANG Li-rong, SUN Xiao-yan, ZHU Ruo-xin, et al. Epidemiological investigation and analysis of women aged 40-55 years old with osteoporosis in Gansu province[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 164 -167 .
[7] CHEN Ru-yue, SHEN Yun-yan, CHEN Qing , et al. Five cases about Henoch-Schönlein purpura complicated with central nervous system injury in children and literatures review[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 168 -171 .
[8] ZHANG Ai-run, FANG Xiao-yi. Lung function testing of bronchopulmonary dysplasia for infants and children[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 172 -176 .
[9] LI Qiu-yu, LI Lu-quan. Risk factors and predictors for aggravation of neonatal necrotizing enterocolitis[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 177 -180 .
[10] HUANG Liu-ming. Development status of precision minimally invasive technique of pediatric surgery[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 190 -192 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed