Journal of Developmental Medicine(Electronic Version)  2014, Vol. 2 Issue (2): 99-103 DOI: |
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| The dynamically changes of Uncoupling protein 2 in myocardial tissue of septic rats |
1Pediatric Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510282 ,China 2Research
Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
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Abstract 【Abstract】Objective To investigate the relationship between uncoupling protein 2(UCP2)expression
and the septic myocardial injury-．Methods Forty SD male rats were randomly divided into 5 groups：control group，6 hour—sepsis group，12 hour—sepsis group，24 hour—sepsis group and 48 hour—sepsis group．The sepsis model was established by intraperitoneal injection of lipopolysaccharide 10 rag／kg．Each group were sac—rificed at the appropriate timepoint．The expression of UCP2 in myocardium was measured by Western Blot—ring．RT—PCR and hnmunohistochemistry-．Results After modeling，UCP2mRNA gradually increased be—ginning at 6 hour—sepsis group，at 24 hour—sepsis group up to the peak value．Sepsis 6，12，24，48 hgroupmyocardial UCP2mRNA was 1．31±0．06，2．94±0．40，8．20±1．96 and 2．15±0．24．The 12，24，48hseptie groups increased significantly compared with that in control group[(0．78±0．22)P<0．05 or P<0．01]．The changes in UCP2 protein levels are similar to mRNA levels．Sepsis 6h，12h，24h，48hgroupsUCP2 protein level was(0．0806±0．0067，0．1063±0．0054，0．1200±0．0057 and 0．0985±0．0096)were significantly higher than those in control group，0．0516±0．0080(P<0．01)．Conclusion septic rat myocardial tissue uncoupling protein 2 up—regulated，this change may be involved in the pathophysiology-of myocardial injury-in sepsis process that requires further study its mechanism．
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Received: 23 January 2014
Published: 05 September 2019
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