Journal of Developmental Medicine(Electronic Version)  2014, Vol. 2 Issue (3): 146-149 DOI: |
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| SET/TAF-1 Regulates the transcription of hematopoietic Genes by Interaction with C/EBPα |
| ZHANG Chi-yuan, ZHU Kang-yong, ZHOU Yong
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| Key Laboratory of Stem Cell Biology and Laboratory ofDevelopment and Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences & Shanghai Jiaotong University School of Medicine, Shanghai 200031, China |
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Abstract 【Abstract】 Objective To investigate the regulatory role of SET/TAF-I in normal and malignant hematopoiesis. Method The expression level of SET/TAF-I was respectively detected in MDS cells, AML cells,lymphocytes and zebrafish embryos by RT-PCR, Western blot and in situ analyses. The interaction between SET/TAF-I and C/EBPα was validated by Co-Immunoprecipitation （Co-IP）. Result SET/TAF-I mRNA expresses in MDS cells, AML cells , lymphocytes as well as caudal hematopoietic tissue （CHT） at 3dpf in zebrafish embryos. SET/TAF-I binds to C/EBPα directly as indicated by Co-IP. Conclusion SET/TAF-I
might regulate the transcription of hematopoietic genes by interacting with C/EBPα.
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Received: 03 July 2014
Published: 05 September 2019
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