Journal of Developmental Medicine(Electronic Version)  2022, Vol. 10 Issue (5): 346-352 DOI: 10.3969/j.issn.2095-5340.2022.05.004 |
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| Effects of low frequency poly-pronuclear on embryo development parameters and clinical pregnancy outcome of patients with in vitro fertilization-embryo transfer treatment |
| Zhu Yuanchang, Yuan Lin, Tan Fang, et al
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(Department of Reproductive Medicine, Shenzhen Hengsheng Hospital, Guangdong, Shenzhen 518034, China)
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Abstract 【Abstract】 Objective To investigate effects of low frequency poly-pronuclear on embryo development
parameters and clinical pregnancy outcome of patients with in vitro fertilization (IVF). Method From
January 1 to December 31, 2020, the infertility patients who underwent in vitro insemination and
embryo transfer (IVF-ET) in the Department of Reproductive Medicine, Shenzhen Hengsheng Hospital
were retrospectively analyzed. A total of 638 cycles with more than 5 eggs obtained were included.
All patients were divided into three groups according to the ratio of poly-pronuclear zygotes: normal
fertilization group, low frequency poly-pronuclear zygotes group (≤10%) and high frequency poly-
pronuclear zygotes group (>10%), the embryo development parameters and clinical pregnancy outcomes
were compared among the three groups. One-way analysis of variance (ANOVA), t-test and χ2-test were
applied as statistical approaches. Result The female average age of low frequency poly-pronuclear
zygotes group was lower than that in normal fertilization group and high frequency poly-pronuclear zygotesgroup [(33±4), (34±5), (34±5) years, F=4.358, P<0.05]. Low frequency poly-pronuclear zygotes
group was higher than the other groups in Anti-millerian hormone levels [(3.8±2.5), (5.1±2.9),
(3.8±3.0) μg/L, F=10.614, P<0.001] and mean number of eggs retrieved [(10.6±5.1), (17.4±6.5),
(11.4±6.4), F=65.357, P<0.001]. High frequency poly-pronuclear zygotes group was higher than
normal fertilization group in high quality embryo rate [44.2% (546/1 235) vs 41.2% (977/2 371),
χ2=1.942, P<0.05]. High frequency poly-pronuclear zygotes group and low frequency poly-pronuclear
zygotes group were higher than normal fertilization group in available blastocyst [64.1% (366/571),
64.8 (529/816), 59.8% (642/1 073)] and high quality blastocysts [(42.4% (242/571), 40.6% (331/816),
33.8% (363/1 073)], which was statistically significant (χ2=3.579, 3.854, 5.982, 5.421, all P<0.05).
Low frequency poly-pronuclear zygotes group were higher than normal fertilization group and high
frequency poly-pronuclear zygotes group in embryo implantation rate [56.4% (31/55), 47.8% (131/274),
47.4%(63/133), χ2=1.273, 1.945, all P<0.05]. Conclusion In IVF cycles, there is a low frequency
(≤10%) of poly-pronuclear insemination, which can obtain a better clinical pregnancy rate and embryo
implantation rate.
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Received: 24 December 2021
Published: 30 September 2022
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