Journal of Developmental Medicine(Electronic Version)  2025, Vol. 13 Issue (1): 26-32 DOI: 10.3969/j.issn.2095-5340.2025.01.004 |
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| Effect of Mycoplasma pneumoniae on airway inflammation based on IL-4/IL-13 signaling pathway |
| Sun Xiaolei, Wang Guowei, Zhou Bin, et al
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(Department of Pediatrics, Huairou Hospital, Beijing Chaoyang Hospital, Capital Medical University, Beijing 101400, China
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Abstract 【Abstract】 Objective To investigate the effect of interleukin (IL) -4/IL-13 signaling pathway on the
differentiation of T helper 2 cell (Th2) and airway inflammation in Mycoplasma pneumoniae pneumonia
(MPP). Method This retrospective study selected 45 children with MPP (including 23 cases in mild
MPP group and 22 cases in severe MPP group) and 18 children with foreign body inhalation (control group)
who were hospitalized in Huairou Hospital, Beijing Chaoyang Hospital, Capital Medical University from
January 2022 to January 2023 as research objects. Bronchoalveolar lavage fluid (BALF) was collected, and
tumor necrosis factor-α (TNF-α), monocyte chemotactic protein 1 (MCP1), IL-6, IL-1β, IL-4, IL-10, IL-
13, IL-5, IL-2, interferon-γ (IFN-γ) levels in BALF was determined using the human cytokine/chemokine
magnetic bead kit. Statistical methods performed by One-way ANOVA analysis, LSD-t test, χ2 test, Fisher
exact probability method and discriminant analysis. Result The C-reactive protein (CRP) and neutrophil
percentage in the severe MPP group were higher than those in the mild MPP group, and the differences werestatistically significant (all P<0.05). The levels of TNF-α, IL-6, IL-1β, MCP1, IL-4, IL-10 and IFN-γ
in BALF in mild MPP group were higher than those in control group. The levels of TNF-α, IL-6, IL-1β,
MCP1, IL-4, IL-10 and IFN-γ in BALF in severe MPP group were higher than those in mild MPP group.
The levels of IL-13 and IL-5 in mild MPP group and severe MPP group were higher than those in control
group, and the differences were statistically significant (all P<0.05). The results of discriminant analysis
showed that 88.9% of the control group, 78.3% of the mild MPP group and 86.4% of the severe MPP groupcould be correctly identified by the cytokine grouping method. The IFN-γ/IL-4 ratios (3.10±1.75, 2.43±1.70 vs 0.97±0.48) and IFN-γ/IL-13 ratios (2.78±1.95, 3.47±2.68 vs 1.00±0.56) in mild and severe MPP groups and control groups showed that the ratios of mild and severe MPP groups were significantly higherthan the control group, and the differences were statistically significant (all P<0.05). Conclusion Mild MPP and severe MPP are characterized by the increase of Th1-related cytokines (IFN-γ) higher than that ofTh2-related cytokines (IL-4 and IL-13), which leads to the increase of BALF inflammation.
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Received: 16 November 2023
Published: 26 January 2025
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