胰岛素样生长因子 -1,早产儿视网膜病变,血管内皮生长因子 ," /> 胰岛素样生长因子 -1,早产儿视网膜病变,血管内皮生长因子 ,"/> Insulin-like growth factor-1,Retinopathy of prematurity,Vascular endothelial growth factor ,"/> <div> <span style="line-height:2;font-size:14px;">外源性补充胰岛素生长因子对氧诱导</span><span style="line-height:2;font-size:14px;">早产儿视网膜病的干预效果</span> </div>
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发育医学电子杂志  2020, Vol. 8 Issue (4): 313-317    DOI: 10.3969/j.issn.2095-5340.2020.04.005
  围产医学   论著 |新生儿 |
外源性补充胰岛素生长因子对氧诱导早产儿视网膜病的干预效果
李晓香  李丽华 李秋平
1. 首都医科大学附属北京潞河医院 儿童中心,北京101100;2. 解放军总医院第七医学中心八一儿童医院 NICU,北京 100700
Interfering effect of exogenous insulin-like growth factor-1 supplementation in oxygen induced retinopathy of prematurity
Li Xiaoxiang , Li Lihua , Li Qiuping
1.Beijing Luhe Hospital, Capital Medical Univercity, Beijing101100, China; 2. Neonatal Intensive Care Unit of Bayi Children's Hospital, Seventh Medical Center of PLA General Hospital, Beijing 100700, China
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摘要 
【摘要】 目的  观察外源性补充重组人胰岛素样生长因子 -1 (human recombinant insulin-like growthfactor-1,rhIGF-1)对氧诱导早产儿视网膜病变(retinopathy of prematurity, ROP)的防治效果。 方法 把生后 7 d 的新生昆明小鼠随机分成 3 组:高氧 rhIGF-1 组、空气对照组和高氧盐水组,每组 16 只。空气对照组在正常环境中饲养,高氧 rhIGF-1 组与高氧盐水组于出生第 7 天(P7)放入 55% ~ 65% 氧浓度的氧箱中饲养,12 d 后出箱,高氧 rhIGF-1 组分别于 P7、P8 下午同一个时间点分别注射 rhIGF-1 一次,每次 0.5 mg/kg ;而高氧盐水组与空气对照组分别注射相同剂量生理盐水,于 P19、P24 各处死 8 只小鼠摘取眼球,ADP 酶染色、视网膜铺片进行观察视网膜上血管的发育情况,并行 HE 染色,观察突破入视网膜内界膜的血管内皮细胞核计数。每天测量各组小鼠体重增长情况。统计学方法采用 t 检验、 ANOVA分析、LSD 多重比较。 结果  比较各组体重增长的情况,空气对照组的体重高于高氧 rhIGF-1 组和高氧盐水组 , 差异均有统计学意义(P<0.05,t 值分别为 3.572,7.553),提示高氧对小鼠体重增长有抑制作用;P19 高氧 rhIGF-1 组小鼠体重增长速度高于高氧盐水组,差异均有统计学意义(P<0.05, t=3.980)。显示rhIGF-1 干预下,能促进高氧环境中小鼠的体重增长。将高氧 rhIGF-1 组与高氧盐水组比较:突破视网膜内界膜的血管内皮细胞核数明显减少,差异有统计学意义(P<0.05);视网膜血管在 P19 走行较自然,周边血管闭塞不明显,周边血管覆盖率高,P24 未见明显新生血管生成。 结论 本研究结果显示外源性补充rhIGF-1 能有效促进小鼠体重的增加,可能减轻高氧对视网膜的损伤,有望成为 ROP 防治的新手段
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关键词:  胰岛素样生长因子 -1')" href="#">    
Abstract: 
【Abstract】 Objective To observe the effect of exogenous supplementation of human recombinant insulin-like growth factor-1 (rhIGF-1) in oxygen-induced retinopathy neonatal mice model. Methods Kunming mice at 7 days after birth were randomly divided into 3 groups: hyperoxia rhIGF-1 group, air control group, and hyperoxia saline group, with 16 mice in each group. Mice in the air control group were assessed to room air. Mice at 7 days after birth in the hyperoxia saline groups and hyperoxia rhIGF-1group were assessed to expose to 55%-65 % oxygen for 12 days . At postnatal day 7 and 8,mice in the hyperoxia rhIGF-1 group received 0.5 mg/kg of rhIGF-1. While air control group and hyperoxia saline group received the same dose of normal saline. At day 19 and 24, 8 mice were put to death, whose eyeballs were extirpated and ADP enzyme staining and stretched preparation of retina for observing retinal blood vessels division. HE dyeing was used for counting retinal vascular endothelial cells. Weightgrowth in each groupof mice was monitored every day. Statistical methods were carried out by t-test, ANOVA analysis and LSDmultiple comparison. Results Body weight gain was compared in each group. Body weight of the controlgroup growed faster than hyperoxia rhIGF-1 group and hyperoxia saline group with statistical significance(P<0.05, t=3.572, 7.553). It showed that hyperoxia has an inhibitory effect on weight gain in mice. P19mice in the hyperoxia rhIGF-1 group gained weight faster than the hyperoxia saline group with statistical
significance (P<0.05, t=3.980). It showed that rhIGF-1 could promote the weight gain of mice in high-oxygen
environment. The hyperoxia rhIGF-1 group was less than hyperoxia saline group in vascular endothelial cells
extending into the inner boundary membrane of retina with statistical significance (P<0.05). Retinal vessel
was normal at P19 and peripheral vascular occlusion was not obvious and peripheral vascular coverage was
high. At P24, neovascularization on retina was not obvious. Conclusion Exogenous complementing of
rhIGF-1 early can effectively promote weight growth of mice, and may mitigate the damage for the retina
caused by hyperoxia, and is expected to be a prophylaxis and treatment method for ROP
Key words:  Insulin-like growth factor-1')" href="#">
收稿日期:  2019-09-18                     发布日期:  2020-11-03     
基金资助: 
国家自然科学基金(81671504)
通讯作者:  李秋平    E-mail:  zhjhospital@163.com
引用本文:    
李晓香  李丽华 李秋平.
外源性补充胰岛素生长因子对氧诱导早产儿视网膜病的干预效果
[J]. 发育医学电子杂志, 2020, 8(4): 313-317.
Li Xiaoxiang , Li Lihua , Li Qiuping.
Interfering effect of exogenous insulin-like growth factor-1 supplementation in oxygen induced retinopathy of prematurity
. Journal of Developmental Medicine(Electronic Version), 2020, 8(4): 313-317.
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http://www.fyyxzz.com/CN/10.3969/j.issn.2095-5340.2020.04.005  或          http://www.fyyxzz.com/CN/Y2020/V8/I4/313
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