胚胎质量,线粒体 DNA,数字聚合酶链式反应," /> 胚胎质量,线粒体 DNA,数字聚合酶链式反应,"/> Embryo quality,Mitochondria DNA,Digital polymerase chain reaction ,"/> <div> <span style="font-size:14px;line-height:2;">胚胎培养液中线粒体 DNA 拷贝数与胚胎</span><span style="font-size:14px;">质量关系的研究</span> </div>
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发育医学电子杂志  2021, Vol. 9 Issue (1): 20-25    DOI: 10.3969/j.issn.2095-5340.2021.01.004
  发育基础   论著 |
胚胎培养液中线粒体 DNA 拷贝数与胚胎质量关系的研究
李亨利  田东梅 侯建文  陈晗笑  朱明辉 许文明  单旭东
1. 成都中医药大学医学与生命科学学院 / 附属生殖妇幼医院 生殖医学中心,四川 成都 610041;2. 四川大学华西第二医院 生殖内分泌与生殖医学调控研究室,四川 成都 610041
Study on the relationship between embryo quality and mitochondrial DNA copies in embryo culture medium
Li Hengli , Tian Dongmei , Hou Jianwen, et al
1. Reproductive Medicine Center, School of Medical and Life Science/Reproductive & Women-Children
Hospital, Chengdu University of Traditional Chinese Medicine, Sichuan, Chengdu 610041, China; 2.
Reproductive Endocrinology and Regulation Laboratory, West China Second University Hospital, Sichuan
University, Sichuan, Chengdu 610041, China
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摘要 
【摘要】 目的  研究第 3 天(day 3,D3)卵裂期胚胎质量与胚胎培养液中线粒体 DNA(mitochondrial
DNA,mtDNA)拷贝数的关系。 方法 选择 2017 年 7 月至 2018 年 10 月于成都中医药大学医学与生
命科学学院 / 附属生殖妇幼医院生殖医学中心行辅助生殖技术助孕的 67 个新鲜卵裂期胚胎移植周期的共 93 个胚胎,应用数字聚合酶链式反应(polymerase chain reaction,PCR)方法检测胚胎培养液中mtDNA 拷贝数。根据形态学总体评价分为优质胚胎组(n=54)和非优质胚胎组(n=39),根据胚胎发育
速度分为正常速度组(n=67)和异常速度组(n=26),根据胚胎评分分为高分组(n=72)和低分组(n=21),比较各组胚胎培养液中 mtDNA 拷贝数,女方年龄、体重指数(body mass index,BMI)、基础内分泌条件及妊娠结局等的差异。采用独立样本 t 检验和秩和检验进行统计学分析。 结果  在其他条件相似的情况下,胚胎培养液中形态学总体评价优质胚胎组平均 mtDNA 拷贝数[(1.30±1.99)copy/μl]明显
高于非优质胚胎组[(0.91±0.94)copy/μl],差异有统计学意义(P<0.05)。胚胎发育正常速度组平均 mtDNA拷贝数[(1.28±1.86)copy/μl]明显高于异常速度组[(0.78±0.71)copy/μl],差异有统计学意义(P<0.05)。胚胎评分高分组 mtDNA 拷贝数[(1.13±1.76)copy/μl]和低分组[(1.15±1.15)copy/μl]比较差异无统计学意义(P>0.05)。 结论 通过数字 PCR 技术检测胚胎培养液中 mtDNA 拷贝数的方法可以作为评价胚胎质量的一种新型非侵入性手段,联合形态学评分与数字 PCR 方法检测胚胎培养液中 mtDNA 拷贝数共同评价胚胎质量的准确性可能更高。胚胎质量越好,其培养液中 mtDNA 拷贝数越高。
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关键词:  胚胎质量')" href="#">胚胎质量  线粒体 DNA  数字聚合酶链式反应    
Abstract: 
【Abstract】 Objective To investigate the relationship between Day 3 embryo quality and mitochondria
DNA(mtDNA)copies in embryo culture medium. Methods From July 2017 to October 2018, 93culture media of transferred embryos from 67 patients who accepeted assisted reproductive technology in
School of Medical and Life Sciences/Reproductive & Women-Children Hospital, Chengdu University of
Traditional Chinese Medicine were selected to assessed mitochondria DNA copies by digital polymerase
chain reaction (PCR) technology. According to the cleavage embryo grading criteria, embryos were divided
into good quality group (n=54) and poor quality group (n=39), normal development rate group (n=67) and abnormal development rate group (n=26), high score group (n=72) and low score group (n=21), respectively.The differences of mtDNA copies, female age, body mass index (BMI), basic endocrine condition andclinical outcome were analysed by t-test or ranks test. Results All the other conditions were nonsignificant except mitochondria DNA copies between groups. The mitochondria DNA copies in embryoculture media of good quality group was significant higher than poor quality group [(1.30±1.99 ) vs(0.91±0.94), P<0.05], normal development rate group was significant higher than abnormal development rategroup [(1.28±1.86) vs (0.78±0.71), P<0.05]. But there was no significant difference between both the highscore group and the low score group of the mitochondria DNA copies in embryo culture media [(1.13±1.76)vs (1.15±1.15) copy/μl, P>0.05]. Conclusions Assessing mitochondria DNA copies by digitalPCR technology in the Day 3 embryo culture medium, in combination with morphological grading,may be a novel, non-invasive, sensitive method to evaluate embryo quality. Good quality and normaldevelopment rate embryo could secrete higher mitochondria DNA copies into embryo culture media.
Key words:  Embryo quality')" href="#">
收稿日期:  2020-11-11                     发布日期:  2021-01-28     
基金资助: 四川省科研院所科技成果转化项目(2017YSZH0032)
通讯作者:  单旭东    E-mail:  Email:chaleswillam@163.com
引用本文:    
李亨利  田东梅  侯建文  陈晗笑  朱明辉  许文明  单旭东.
胚胎培养液中线粒体 DNA 拷贝数与胚胎质量关系的研究
[J]. 发育医学电子杂志, 2021, 9(1): 20-25.
Li Hengli , Tian Dongmei , Hou Jianwen, et al.
Study on the relationship between embryo quality and mitochondrial DNA copies in embryo culture medium
. Journal of Developmental Medicine(Electronic Version), 2021, 9(1): 20-25.
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