Abstract: 【Abstract】 Objective To detect the expression levels of interleukin-6 (IL-6), interleukin-10 (IL- 10), tumor necrosis factor (TNF-α) and C-reactive protein (CRP) in purulent meningitis (PM) caused by group B streptococcus (GBS) infection in neonates, and to discuss their clinical value. Method 59 PM newborns with GBS infection born in the First Hospital of Qinhuangdao from May 2017 to May 2020 were included in the observation group, and 59 PM newborns without GBS infection (late-onset sepsis) were included in the control group during the same period. The levels of serum vitamin D, CRP, IL-6, IL-10 and TNF-α were detected in all subjects, and Pearson correlation analysis was performed. The diagnostic value of serum vitamin D and inflammatory cytokines in PM neonates with GBS infection was analyzed by receiver operating characteristic (ROC) curve. The statistical methods performed by t-test, χ2 test and Pearson correlation analysis. Result The pregnant women in the observation group had premature rupture of fetal membrane [47.5% (28/59) and 5.1% (3/59), χ2=27.345], asphyxia during labor [52.5% (31/59) vs 18.6% (11/59)] and [(44.1% (26/59) vs 22.0% (13/59), χ2=6.473] was significantly higher than that of the control group (P<0.05). Serum vitamin D level in the observation group was significantly lower than that in control group [(13.3±2.1)μg/L vs (21.1±5.0) μg/L, t=11.345]. The observation group were significantly higher than those in control group in IL-6 [(87.1±14.5) μg/Land (63.9±11.9) μg/L, t=9.507], IL-10 [(49.6±15.2) μg/L and (29.3±10.0) μg/L, t=8.596], the TNF-α [(76.8±19.0) μg/L vs (50.0±10.8) μg/L,t=9.410] and CRP [(21.5±5.0) μg/L vs (13.7±3.7) μg/L, t=9.702] (P<0.05). The Pearson correlation analysis results showed that the serum vitamin D level in the observation group was negatively correlated with the levels of IL-6, IL-10, TNF-α and CRP, respectively (r=-0.662, -0.644, -0.564, -0.643, P<0.05). The areaunder the curve (AUC) of serum vitamin D, IL-6, IL-10, TNF-α and CRP in the alone diagnosis of neonatalPM with GBS infection were 0.831 (95% CI: 0.757-0.904), 0.887 (95% CI: 0.830-0.944), 0.859 (95% CI:0.793-0.925), 0.888 (95% CI: 0.821-0.955) and 0.879 (95% CI: 0.820-0.938), respectively, and the AUCof five combined tests was 0.991 (95% CI: 0.978-1.000). Conclusion Serum vitamin D levels of PM neonates caused by GBS infection decreased and inflammatory cytokine levels increased, which has certain auxiliary diagnostic value for PM caused by GBS infection.
乔木 韩雁雁 姚文秀. 新生儿GBS感染所致化脓性脑膜炎中血清维生素D和炎性细胞因子的表达及意义[J]. 发育医学电子杂志, 2024, 12(2): 96-101.
Qiao Mu, Han Yanyan, Yao Wenxiu. Expression and significance of serum vitamin D and inflammatory cytokines in neonatal purulent meningitis caused by GBS infection. Journal of Developmental Medicine(Electronic Version), 2024, 12(2): 96-101.
2024, Vol. 12 
