Abstract: 【Abstract】 Objective To explore the application value of quantitative determination of urinary gonadotropin(Gn), including urinary luteinizing hormone (LH) and urinary follicle stimulating hormone (FSH) in judging thesexual development and the diagnose of central precocious puberty (CPP) in girls. Method From December2021 to October 2022, 72 girls who were hospitalized in the Department of Endocrinology and Metabolismin Children, Anhui Provincial Children's Hospital due to breast development were selected by retrospectivestudy. Based on the results of the gonadotropin-releasing hormone (GnRH) stimulation test, these childrenwere divided into two groups: CPP group (n=48) and premature thelarche (PT) group (n=24). The morningurinary Gn concentration, random urine Gn concentration within 2 hours post-GnRH stimulation test, andblood Gn concentration associated with the GnRH stimulation test were analyzed, and the levels of LH andFSH in blood and urine along with related parameters between two groups were compared and analyzed.Statistical methods performed by t-test, Mann-Whitney U tests, Spearman correlation analysis, and receiveroperating characteristic (ROC) curve analysis. Result Urinary FSH concentrations post-stimulation inCPP group were significantly lower than that in PT group [31.09 (24.21, 39.18) mIU/ml vs 45.67 (30.78,60.93) mIU/ml, Z=-2.230, P<0.05]. Morning urinary and post-stimulation urinary LH, as well as morningurinary and post-stimulation urinary LH/FSH in CPP group were 1.27 (0.66, 3.57) mIU/ml, 9.44 (4.58, 20.68)mIU/ml and 0.07 (0.04, 0.15), 0.22 (0.15, 0.50). Meanwhile, the corresponding indexes in PT group were 0.77(0.60, 1.00) mIU/ml, 3.71 (1.85, 6.07) mIU/ml and 0.04 (0.03, 0.06), 0.08 (0.06, 0.15). The expression levelsof the four indicators in CPP group were higher than those in PT group (all P<0.05). Spearman correlationanalysis showed that morning urinary LH was positively correlated with the blood LH basal value (r=0.645,P<0.05) and urinary LH after stimulation (r=0.347, P<0.05), urinary LH after stimulation was positivelycorrelated with the blood LH peak value (r=0.385, P<0.05), and urinary LH/FSH after stimulation waspositively correlated with blood LH/FSH peak ratio (r=0.393, P<0.05). ROC curve analysis showed that whenthe morning urinary LH was≥1.12 mIU/ml, the sensitivity and specificity of diagnosis for CPP were 60.0% and86.4%. When the urinary LH was≥8.23 mIU/ml after stimulation, the sensitivity and specificity of diagnosis forCPP were 53.3% and 95.7%. Conclusion The detection of urinary Gn can be used as a non-invasive and simple diagnostic method, which has certain significance in judging the sexual development of girls.
余丹 邓茜 王娟娟 高健 陈雨青. 定量测定尿促性腺激素判断女童性发育的临床应用[J]. 发育医学电子杂志, 2025, 13(1): 56-61,65.
Yu Dan, Deng Qian, Wang Juanjuan, et al. Clinical application of quantitative determination of urinary gonadotropin in judging the sexual development in girls. Journal of Developmental Medicine(Electronic Version), 2025, 13(1): 56-61,65.
2025, Vol. 13 
