发育医学电子杂志 Vol. 5 28-34
发育基础
综述
单细胞转录组数据分析与发育生物学 研究进展
李品1,2 贺宁1,3 李艳明1,3 方向东1,2,3
1.中国科学院北京基因组研究所 基因组科学与信息重点实验室，北京 100101；
2.中国科学院大学 生命科学院，北京 100049；
3.中国科学院大学 中丹学院，北京 100190
Analyses of single-cell RNA sequencing data in developmental biology studies
摘要 细胞的发育是一个动态过程，染色质状态的变化、DNA表达等分子层面的调控支撑着细胞形态的变化和功能的完善。转录组测序技术（RNA-sequencing method，RNA-seq）是研究细胞发育的重要手段之一，能够获得细胞发育不同阶段全基因组的表达信息；但其结果只是细胞群基因表达量变化的均值，忽略了细胞的异质性。单细胞转录组测序技术（single-cell RNA-sequencing method，scRNA-seq）可以在单细胞水平获得全基因组范围的表达谱，有助于发现罕见细胞类型，并能更直观反映处于不同生长发育阶段的细胞中基因表达变化。虽然scRNA-seq已经取得了很多有意义的结果，但是该项技术操作还面临挑战；另外，scRNA-seq数据的处理与常规RNA-seq也有所区别，目前已经逐渐发展出针对scRNA-seq的计算机技术和算法。
关键词:
单细胞测序
转录组
数据分析
收稿日期: 2016-11-16
出版日期: 2017-01-30
发布日期: 2018-01-19
期的出版日期: 2017-01-30
基金资助: 国家自然科学基金（31471115，31401160，81670109）
通讯作者:
方向东
E-mail: fangxd@big.ac.cn
引用本文:
李品 贺宁 李艳明 等. 单细胞转录组数据分析与发育生物学 研究进展 [J]. 发育医学电子杂志, 2017, 5(1): 28-34.
Analyses of single-cell RNA sequencing data in developmental biology studies . Journal of Developmental Medicine(Electronic Version), 2017, 5(1): 28-34.
链接本文:
http://www.fyyxzz.com/CN/
或
http://www.fyyxzz.com/CN/Y2017/V5/I1/28
[1]
GAO Xiao-hui, MAO Jian. Clinical features of non-oliguric hyperkalemia in extremely low birth weight infants
[2]
Society of Neonatologist, Chinese Medical Doctor Association. Consensus recommendations on the prevention and early management of respiratory distress syndrome in preterm infants
[3]
Professional Committee of Respiratory, Society of Neonatologist, Chinese Medical Doctor Association. Clinical application recommendations for heated humidified high flow nasal cannula
[4]
YAN Jun, ZHU Xing-wang, SHI Yuan. Application progress of noninvasive ventilate technique for premature infants
[5]
GU Min-fang, YANG Chuan-zhong. Progress of intrapartum resuscitation for premature infants
[6]
LIU Shu-hua, SHEN Yue-bo, LIU Cui-qing, MA Li. The efficacy of pulmonary surfactant for pulmonary function in premature tension pneumothorax
[7]
GAO Xiao-hui, MAO Jian. Clinical features of non-oliguric hyperkalemia in extremely low birth weight infants
[8]
XIA Yao-fang, YANG Juan , TIAN Bao-li, et al. Value of amplitude-integrated electroencephalography in monitoring acute period of neonatal bilirubin encephalopathy and prognostic assessment
[9]
WANG Li-rong, SUN Xiao-yan, ZHU Ruo-xin, et al. Epidemiological investigation and analysis of women aged 40-55 years old with osteoporosis in Gansu province
[10]
CHEN Ru-yue, SHEN Yun-yan, CHEN Qing , et al. Five cases about Henoch-Schönlein purpura complicated with central nervous system injury in children and literatures review
Viewed
Full text
Abstract
Cited
Shared
Discussed
2017, Vol. 5 
