human embryos at 7, 10, 12 and 16 weeks of age were compared with normal adult inner ear and congenital inner ear malformations. High-resolution 2D images of human embryo specimens were obtained by digital slice scanning. The inner ear structure of human embryo and adult was measured at different gestation weeks. Mimics 21.0 software was used for 3D modeling of membrane labyrinth.　Results　The structure of the inner ear were clearly distinguished in high-definition 2D images after digital slice scanning and 3D reconstruction of histological images were clearly than MRI imaging. The angles of the anterior and posterior semicircular canal common membrane feet of the 6 groups, each of 7, 10, 12, 16-week, normal adults and inner ear deformities was 157.36o, 155.19o, 153.79o, 150.58o, 121.36o and 121.88o. The cochlea curved median side of vestibule, each angle was 130.15o, 120.68o, 146.12o, 112.73o, 104.61o and 121.88o, respectively. Except of patients with inner ear malformation, the angles between the plane of the lateral semicircular canal and the horizontal axis of the skull were 36.86o, 21.08o, 18.37o, 15.25o and 15.65o. The three semicircular canals of the inner ear of 7, 10, 16-week and adults were vertical to each other. The angle between the anterior semicircular canal and the posterior and lateral semicircular canals in 12-week was less than 90o. The spiral tube around the worm axis in specimen were formed 2.5 circles at 16 weeks and 12 weeks, 2 circles at 10 weeks, and less than 1 circle at 7 weeks. In patients with congenital inner ear malformations, the size of the vestibular cystic dilation was about 6.1 mm x 4.6 mm, and the lateral semicircular canal was unclear.　Conclusions　Different methods are used to reconstruct the labyrinth of the inner ear during the development stage and after birth, which can reflect the spatial position of the inner ear structures and obtain the relevant anatomical

measurement values, so we can predict the developmental stagnation time of congenital inner ear malformation in the embryonic stage, providing a preliminary basis for the diagnosis of early deafness in infants.