Listeria monocytogenes (LM) is a significant pathogen responsible for severe infections in neonates, leading to critical conditions such as sepsis and meningitis, which are associated with high rates of mortality and disability. Early and accurate laboratory diagnosis is essential for timely intervention and improving neonatal outcomes. This article provides a systematic review of the primary laboratory diagnostic methods for neonatal LM infection, encompassing traditional culture techniques, serological testing, molecular biological technologies, and emerging diagnostic approaches. It further explores the current challenges in the diagnostic process, aiming to offer insights for optimizing laboratory diagnostic strategies for neonatal LM infection.
2026, Vol. 14 
