发育医学电子杂志  2018, Vol. 6 Issue (3): 149-153 DOI: 10.3969/j.issn.2095-5340.2018.03.004 |
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发育基础
专题笔谈
｜新生儿
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| piggyBac 转座系统的发展及应用 |
| 张文豪 李旭 帅领 |
| 1. 南开大学 药物化学生物学国家重点实验室，天津 300350；2. 南开大学 药学院，天津 300350 |
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| The application and development of piggy Bac transposon system |
| ZHANG Wen-hao, LI Xu , SHUAI Ling |
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摘要 转座子是指在原核和真核生物基因组上可以进行位置转换的 DNA 片段。转座子通过在原始位置上进行剪切或复制，经环化作用在转座酶辅助下插入到宿主基因组的其他位置，转座子发生位置转移的过程即为转座。转座子可以在基因组任意插入和切除，常用于哺乳动物细胞基因编辑的研究之中。相较于病毒介导的转染和传统转座子系统，piggyBac（PB）转座子拥有多种优点，广泛应用于转基因及功能基因研究中。本文对转座子系统的发
展历史、PB 转座子的结构特点及最新研究进展进行阐述。
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| 关键词:
转座子 ')" href="#">转座子
细胞治疗
靶向基因编辑技术
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收稿日期: 2018-03-25
出版日期: 2018-07-30
发布日期: 2018-08-20
期的出版日期: 2018-07-30
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| 基金资助: 国家自然科学基金面上项目（31671538）；国家自然科学基金青年项目（31501186）；天津市自然科学基金重点项目（15JCZDJC65300） |
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通讯作者:
帅领http://pharmacy.nankai.edu.cn/teamd.aspx?id=106
E-mail: lshuai@nankai.edu.cn
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