microRNome analysis of the Zebrafish model of diamond-blackfan anemia from Rps19 deficiency
ZHANG Qian, SONG Bin-feng, WAN Yang,et al
1. CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing100101, China; 2. Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan 430074, China; 3. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China ）
Abstract: 【Abstract】 Objective To characterize the genome-wide microRNA profile and to explore the biological functions and probable mechanisms for microRNAs in diamond-blackfan anemia （DBA）. Method Zebrafish embryos at the one-cell stage were injected with the Rps19 MO and Rps19+p53 MO. The miRNomes of these zebrafish models and control, generated by miRNA-seq, were compared for determining the microRNA profile characteristics of DBA and regulatory function of p53. Result Rps19 morphants showed an obviously ventrally bent tail and a reduction in the circulating blood cells compared with the control embryos, which werepartially rescued in Rps19+p53 MO at 48 hpf. By comparing the miRNomes with control, we found that about25% of total expressed microRNAs in Rps19 MO had significantly lower expression, which might make an influence on the development process of Zebrafish embryos. The abnormal miRNomes were partially reversed by simultaneous knockdown of p53. We determined 47 microRNAs that were potentially regulated by p53 in DBA pathologic process.Conclusion microRNAs in Zebrafish model of DBA were in an inactive state, which was partially affected by p53.
张倩 宋斌峰 万扬 张昭军 鲍丙浩 谢尚论 刘汉芝 竺晓凡 袁卫平 . 先天性纯红细胞再生障碍性贫血斑马鱼模型相关microRNA的组学研究[J]. 发育医学电子杂志, 2014, 2(3): 139-145.
ZHANG Qian, SONG Bin-feng, WAN Yang, et al. microRNome analysis of the Zebrafish model of diamond-blackfan anemia from Rps19 deficiency. Journal of Developmental Medicine(Electronic Version), 2014, 2(3): 139-145.
2014, Vol. 2 
