发育医学电子杂志 Vol. 2 146-149
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论著
SET/TAF-I通过C/EBPα调控 造血系统基因转录
张驰原 朱康勇 周勇
中国科学院干细胞重点实验室，中国科学院上海生命科学 研究院/上海交通大学医学院健康科学研究所，发育与疾病实验室，上海 200031
SET/TAF-1 Regulates the transcription of hematopoietic Genes by Interaction with C/EBPα
ZHANG Chi-yuan, ZHU Kang-yong, ZHOU Yong
Key Laboratory of Stem Cell Biology and Laboratory of Development and Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences & Shanghai Jiaotong University School of Medicine, Shanghai 200031, China
摘要 【摘要】 目的 研究SET/TAF-I 基因调控造血系统基因转录的机制，揭示SET/TAF-I 基因异常导致疾病的原因。方法 应用RT-PCR、Western blot 和原位杂交技术，检测造血细胞、白血病细胞和 斑马鱼胚胎中SET/TAF-I 基因的表达水平；应用免疫共沉淀技术，检测SET/TAF-I 与C/EBPα 的相 互作用。结果 SET/TAF-I 基因在骨髓增生异常综合征（MDS）细胞、急性髓系细胞白血病（AML） 细胞和淋巴细胞中，以及斑马鱼胚胎尾部造血组织中都有富集表达；SET/TAF-I 和C/EBPα 间存在相 互作用。结论 C/EBPα 是SET/TAF-I 靶基因，SET/TAF-I 通过C/EBPα 调控下游造血系统基因的转录。
关键词:
SET/TAF-I ')" href="#">SET/TAF-I
C/EBPα
造血
白血病
斑马鱼
Abstract: 【Abstract】 Objective To investigate the regulatory role of SET/TAF-I in normal and malignant hematopoiesis. Method The expression level of SET/TAF-I was respectively detected in MDS cells, AML cells, lymphocytes and zebrafish embryos by RT-PCR, Western blot and in situ analyses. The interaction between SET/TAF-I and C/EBPα was validated by Co-Immunoprecipitation （Co-IP）. Result SET/TAF-I mRNA expresses in MDS cells, AML cells , lymphocytes as well as caudal hematopoietic tissue （CHT） at 3dpf in zebrafish embryos. SET/TAF-I binds to C/EBPα directly as indicated by Co-IP. C onclusion SET/TAF-I might regulate the transcription of hematopoietic genes by interacting with C/EBPα.
Key words:
SET/TAF-I ')" href="#">SET/TAF-I
C/EBPα
Hematopoiesis
Leukemia
Zebrafish
收稿日期: 2014-07-03
出版日期: 2014-07-30
发布日期: 2019-09-05
期的出版日期: 2014-07-30
通讯作者:
周勇
E-mail: zhouyong@sibs.ac.cn
引用本文:
张驰原 朱康勇 周勇. SET/TAF-I通过C/EBPα调控 造血系统基因转录 [J]. 发育医学电子杂志, 2014, 2(3): 146-149.
SET/TAF-1 Regulates the transcription of hematopoietic Genes by Interaction with C/EBPα . Journal of Developmental Medicine(Electronic Version), 2014, 2(3): 146-149.
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