Establishment of linear thermal injury-induced deep partial-thickness burn wound models in mice dorsal skin
Liu Xuelai, Li long, Li Suolin, et al
1.Department of Surgery, Children’s Hospital Capital Institute of Pediatric, Beijing 100020, China; 2.Department of Pediatric Surgery, the Second Hospital of Hebei Medical University, Hebei, Shijiazhuang 050000, China; 3.Department of Central Laboratory, the Second Hospital of Hebei Medical University, Hebei,Shijiazhuang 050000, China; 4.Department of Endocrine, Jilin Provincial People's Hospital, Jilin, Changchun130012, China; 5.Department of Animal Experiment, the Second Hospital of Hebei Medical University, Hebei,Shijiazhuang 050000, China; 6.Department of Surgery, Yinchuan Women and Children Healthcare Hospital,Ningxia, Yinchuan 750001, China; 7.Department of Surgery, Tianjin Children Hospital, Tianjin 300070, China)
Abstract: 【Abstract】 Objective To introduce and describe establishment of linear thermal injury-induced deep partial-thickness burn wound models in mice dorsal skin, and to observe the morphology of wound edge and injury depth, as well as investigate its feasibility and manipulability. Methods Balb/C mice aged 10-12 weeks were obtained for anesthesia and dorsal skin was exposed after shaving, which were fixed to the operating platform by prone position. The high frequency electrosurgical scalpel plate curled around the tail 15-18 J working energy).The whole skin of the mice back was lifted with tweezers, and the needle type electric knife tip was inserted into the skin to draw a 1 cm long incision uniformly and longitudinally. The wound was covered with sterile gauze. The mice were sacrificed by breaking neck at 12 h after operation.Full-layer skin specimens (wound area: 1.0 cm×1.0 cm) were collected from the scalded area, followed by 4% paraformaldehyde fixation, dehydration, embedding, sectioning (5 μm thickness), which was close to the broken edge of linear scald tissue. Histological structure was observed by HE staining. ResultsHE staining showed that linear wound could accurately injury the whole skin layer and depth of burn was as deep as "dermal reticular layer and part of subcutaneous tissue layer" including muscular layer in skin through microscopical observation. Cross-sectional morphological observation showed that coagulation necrosis of tissue in epidermis and dermal reticular layer caused by instantaneous energy release by scald. The hair bud and hair follicle in epidermis disappeared and vacuole deformation were observed in dermis,partial accumulation of skin muscle layer. Breakage of muscle fibers in dermis, hypertrophy with infiltration of inflammatory cells were observed. Conclusion The use of high frequency electrosurgical scalpel is an easy and reliable, short modeling period and practical advantages approach to establish of linear thermal injury-induced burn wound models in mice dorsal skin, presenting stable controlling and accurate deep partial-thickness in tissue injury and depth.
刘雪来 李龙 李索林 宋岩彪 杜娟 靳晓次 郑颖龙 费川 张永 婷. 采用电切法建立小鼠皮肤线形深Ⅱ°烫伤模型[J]. 发育医学电子杂志, 2021, 9(4): 259-262.
Liu Xuelai, Li long, Li Suolin, et al. Establishment of linear thermal injury-induced deep partial-thickness burn wound models in mice dorsal skin. Journal of Developmental Medicine(Electronic Version), 2021, 9(4): 259-262.
2021, Vol. 9 
