Experimental research on inflammatory response around silk knots in abdominal wall induced by de-wax silk and conventional silk
LIU Xue-lai SONG Yan-biao SHAN Ying-jun ZHANG Chuang JIN Xiao-ci4 FEI Chuan ZHANG Yong-ting LI Suo-lin
1.Department of Pediatric Surgery, The Second Hospital of Hebei Medical University, Hebei, Shijiazhuang 050000, China; 2.Department of Central Laboratory, The Second Hospital of Hebei Medical University, Hebei, Shijiazhuang 050000, China; 3.Department of Urinary Surgery, China-Japan Friendship Hospital, Beijing 100029, China; 4.Department of Animal Experiment, The Second Hospital of Hebei Medical University, Hebei, Shijiazhuang, 050000, China
Abstract: 【Abstract】 Objective To explore the early postoperative in?ammatory cells in?ltration around silk knots and delayed-type hypersensitivity induced by conventional silk and de-wax silk in abdominal wall of mice, and to compare the differences of the two silks. Methods The study was implemented from November 2017 to January 2018. Experimental animals were 12 Balb/C mice. Totally 9 mice were assigned to the conventional silk group and knotted by sterile 4-0 silk with needle. Then they were randomly divided into three subgroups, including subcutaneous group, intramuscular group and extra peritoneal space group, three mice of each group. The knot segments were cut off on day 7 post-operation. Meanwhile, three mice for de-wax silk group were knotted in extra peritoneal space with de-wax silk, then tissue was cut off on day 5 post-operation. HE and immunohistochemistry (IHC) staining were engaged to compare the differential hypersensitivity around silk knots between conventioal and de-wax group. Results There were no obvious foreign body reactions and histological lumps induced by knotting through the naked eye in all groups. HE staining indicated that the knots were created accurately in different anatomical locations of abdominal wall. The IHC result on day 7 post-operation of the conventional silk group indicated that no macrophage (F4/80 markers) was infiltrated and no expression of inflammatory protein (IL-6 and TNF-α markers) was noted in subcutaneous group, intramuscular group and extra peritoneal space group. While the IHC result on day 5 post-operation of the de-waxed silk group indicated that there was signi?cant macrophage in?ltration, expression of in?ammatory protein, MHC Ⅰ / Ⅱ and CD4 in?ltration, extracellular matrix produced by antigen presenting cells (APC) and T-cells around silk knots in extra peritoneal space, indicating that delayed-type hypersensitivity happened. Conclusions The de-wax silk knotted in extra peritoneal space of mice abdominal wall induces delayed-type hypersensitivity and foreign body in?ammatory response. Conventional silk may delay the foreign body in?ammatory reaction due to the surface with wax.
2018, Vol. 6 
