Abstract: 【Abstract】 Objective To investigate the copy number variations of Klebsiella in feces of preterm infantswith feeding intolerance. Method From April 2022 to April 2024, premature infants with gestational age of 28 to 37 weeks who were admitted to the Department of Pediatrics, Qingpu Branch of Zhongshan HospitalAffiliated to Fudan University with hospitalization time ≥14 d were included in the study by prospectivestudy method. After hospitalization, the premature infants received conventional treatment and was monitoredfor feeding intolerance. Fecal samples were collected at 3, 7, and 14 days of age, and the copy numberof Klebsiella in the feces was detected using probe-based fluorescent quantitative polymerase chainreaction (PCR) technology. The statistical methods performed by one-way ANOVA, Scheffe method, χ2test and χ2 test segmentation. Result A total of 128 premature infants were included in this study,and the incidence of feeding intolerance gradually decreased in the extremely preterm infants group(n=32), the moderately preterm infants group (n=58) and the late preterm infants group (n=38), and thedifference was statistically significant (P<0.05). The extremely preterm infants group (n=17) with feedingintolerance at 7 and 14 days of age were compared with the moderately preterm infants group (n=9) andthe late preterm infants group (n=1), and the difference was statistically significant (P<0.05). Among thethree gestational age groups, the infants with 7 days of age had higher fecal Klebsiella copy numbers,with a gradual decrease observed in the extremely preterm, moderately preterm and late preterm groups[(264.91±77.62) copies/μl, (195.02±96.23) copies/μl, (138.82±61.47) copies/μl, P<0.001]. In theextremely preterm infant group, the fecal Klebsiella copy numbers in infants at 7 days and 14 days of agewere significantly higher than those in the moderately preterm group and the late preterm infant group [7 daysof age: (264.91±77.62) copies/μl vs (195.02±96.23) copies/μl, (138.82±61.47) copies/μl, P values were0.010 and P<0.001, respectively; 14 days of age: (123.69±62.23) copies/μl vs (88.23±50.21) copies/μl,(81.55±47.49) copies/μl, P values were 0.040 and 0.030, respectively]. A comparison of fecal Klebsiella copynumbers between the feeding intolerance group (n=79) and the feeding in tolerance group (n=49) showedthat the fecal Klebsiella copy numbers in infants with feeding intolerance were significantly higher at both7 days and 14 days of age [7 days of age: (297.44±83.19) copies/μl vs (261.29±72.04) copies/μl; 14 daysof age: (169.81±62.77) copies/μl vs (133.29±51.33) copies/μl, P values were 0.020, 0.002]. The copy numbersof Klebsiella in the feces of infants with feeding intolerance were significantly higher than those with feeding tolerancegroup in the extremely preterm, moderately preterm, and late preterm groups (227.67±75.40) copies/μl vs (195.02±50.81) copies/μl, (184.90±43.24) copies/μl vs (157.05±32.30) copies/μl, and (123.99±29.29) copies/μl vs (102.78±22.32) copies/μl, respectively, P values was 0.014, <0.001, <0.001).Conclusion The copynumbers of Klebsiella in the feces of premature infants is related to feeding intolerance. The copy numbers ofKlebsiella in feces of premature infants decreased with the decrease of feeding intolerance. The copy numbersof Klebsiella in feces of premature infants with feeding intolerance increased significantly
贾西燕 徐灵敏 郭荣荣 唐利芳 王忻宇. 喂养不耐受早产儿粪便克雷伯杆菌DNA拷贝数变化分析[J]. 发育医学电子杂志, 2025, 13(3): 182-187.
Jia Xiyan, Xu Lingmin, Guo Rongrong, et al. Analysis of DNA copy number variations of Klebsiella in feces of preterm infants with feeding intolerance. Journal of Developmental Medicine(Electronic Version), 2025, 13(3): 182-187.
2025, Vol. 13 
