基于Open Array 技术的妊娠期阴道菌群检测研究

doi:10.3969/j.issn.2095-5340.2021.03.005

发育医学电子杂志

2021, Vol. 9

Issue (3): 188-193 DOI: 10.3969/j.issn.2095-5340.2021.03.005

围产医学论著｜产科

基于Open Array 技术的妊娠期阴道菌群检测研究

郭琦　庄彩霞　蒋宇林　刘俊涛　肖盟　刘理达

1. 中国医学科学院北京协和医学院／北京协和医院　产科中心，北京100730；2. 首都医科大学附属
北京朝阳医院　妇产科，北京　100020；3. 中国医学科学院　北京协和医学院／北京协和医院　检验科／北京市重点实验室，北京　100730；4. 北京市平谷区医院妇产科，北京　101200）

A study of vaginal microflora detection during pregnancy based on Open Array technology

Guo Qi, Zhuang Caixia, Jiang Yulin, et al

(1. Department of Obstetrics,Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100730, China; 2.Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, Beijing100020, China; 3. Department of Laboratory , Beijing Key Laboratory , Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100730, China; 4. Obstetrics and Gynecology, Beijing Pinggu Hospital, Beijing 101200, China)

摘要
相关文章
推荐阅读
Metrics

下载:

PDF (1065KB)
输出: BibTeX | EndNote (RIS)

摘要 【摘要】目的　探讨 Open Array 平台技术用于孕期阴道菌群分析及筛查感染病原菌检测的临床效率。
方法　选择2017 年11 月至2018 年5 月在北京协和医院产检的孕妇中纳入200 例。采集其阴拭子标本，
分别应用传统培养法和Open Array 平台技术进行检测。　结果　对于阴道真菌和细菌的检测，传统培
养法和Open Array 平台技术的检测阳性率分别为19.0%（38/200）和20.0%（40/200），差异无统计学意
义（P=0.800）。传统培养法和Open Array 平台技术检测细菌的总相符率为94.5%，真菌相符率为97.1%。
此外，Open Array 平台技术可另检出细菌性阴道病（bacterial vaginosis，BV）34 例（17.0%）。本研究中单纯BV 阳性病例中，胎膜早破比例（26.1%）较BV 阴性且无其他感染的病例中发生率（17.6%）稍高，但尚不具有显著性差异（P=0.053），需进一步的大样本量研究。　结论　Open Array 平台技术可以作为筛查妊娠期阴道菌群及病原体的快速检测技术，与传统培养的检出率相当，符合度较高，在临床需要快速检
测进行针对性处理及用药指导时具有可信性。

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

关键词: 培养法')" href="#">培养法相符率细菌性阴道病妊娠期 Open Array 技术

Abstract: 【Abstract】 Objective To investigate the clinical efficiency of Open Array platform technology in the analysis of vaginal flora during pregnancy and the detection rate of pathogens screening. Methods　From November 2017 to May 2018, 200 pregnant women in Peking Union Medical College Hospital were enrolled, whose swab specimens were tested by traditional culture and Open Array platform technology.　Results　For the detection of vaginal candidiasis and bacteria infection, the positive rates of traditional culture method and Open Array platform technology were 19.0% (38/200) and 20.0% (40/200), respectively, with no statistical difference (P=0.800). The accordance rate between traditional culture and Open Array platform technology for candida and bacteria was 94.5%, and 97.1% for candidasis. In addition, 34 cases (17.0%) of bacterial vaginosis (BV)were detected by Open Array platform. In this study, the proportion of premature rupture of membranes in BV positive cases (26.1%) was higher than that in BV negative or any other infection cases (17.6%), but there was no significant difference (P=0.053), and further study with a large sample size was needed.Conclusions　Open Array platform technology can be used as a rapid detection technology for screening vaginal flora and pathogens during pregnancy. The detection rate of Open Array platform technology is similar to that of traditional culture with a high degree of conformity. It has credibility when there are clinical needs for rapid detection for targeted treatment and medication guidance.

Key words: Culture method')" href="#">Culture method Accordance rate Bacterial vaginosis Pregnancy Open Array technology

收稿日期: 2021-02-19 发布日期: 2021-05-26

通讯作者: 蒋宇林 E-mail: Jiangyulin@pumch.cn

引用本文:

郭琦　庄彩霞　蒋宇林　刘俊涛　肖盟　刘理达. 基于Open Array 技术的妊娠期阴道菌群检测研究[J]. 发育医学电子杂志, 2021, 9(3): 188-193.
Guo Qi, Zhuang Caixia, Jiang Yulin, et al. A study of vaginal microflora detection during pregnancy based on Open Array technology. Journal of Developmental Medicine(Electronic Version), 2021, 9(3): 188-193.

链接本文:

http://www.fyyxzz.com/CN/10.3969/j.issn.2095-5340.2021.03.005 或 http://www.fyyxzz.com/CN/Y2021/V9/I3/188

[1]	金玉　严滨　关怀. 妊娠合并系统性红斑狼疮的管理[J]. 发育医学电子杂志, 2021, 9(4): 313-316.
[2]	胡凌云　谢潇潇　李立安　徐亚君　王黎　周飞虎　郭旭　游艳琴　卢彦平. 产褥感染的病原菌分布及妊娠结局分析[J]. 发育医学电子杂志, 2021, 9(4): 282-286.
[3]	王方　郑剑兰　汪文雁　彭芳林. 辅助生殖技术与妊娠期糖尿病孕产妇肠道菌群的特异表达[J]. 发育医学电子杂志, 2021, 9(2): 114-121.
[4]	曲冬颖　陈佳　韩悦　孙静莉. 妊娠期高脂血症孕妇高密度脂蛋白抗炎抗氧化功能的变化[J]. 发育医学电子杂志, 2021, 9(2): 109-113.
[5]	陈晗笑王翔　张曜耀　许文明　何国琳　秦朗. 维生素 D 在胎盘滋养层的代谢及其对孕妇和子代的保护作用[J]. 发育医学电子杂志, 2021, 9(1): 8-13,19.
[6]	刘艳　王政禄. 妊娠期高血压疾病胎盘样本库的建设与管理 [J]. 发育医学电子杂志, 2020, 8(4): 373-377.
[7]	梁杨　么大伟. 妊娠期糖尿病孕妇脂联素、趋化素、超敏C反应蛋白及胰岛素抵抗的表达及意义[J]. 发育医学电子杂志, 2019, 7(4): 282-286.
[8]	何素健　刘喜红　杨波　罗红凤　黎常红　肖妮蓉　徐明枝. 妊娠期糖尿病产妇母乳n-3多不饱和脂肪酸含量对婴儿早期生长发育的影响[J]. 发育医学电子杂志, 2019, 7(4): 274-281.
[9]	柯华　李占魁. 母亲妊娠期高血压疾病对新生儿预后的影响[J]. 发育医学电子杂志, 2018, 6(2): 74-76.
[10]	李佳　侯新琳. 应用振幅整合脑电图评价妊娠期糖尿病对新生儿脑发育的影响[J]. 发育医学电子杂志, 2016, 4(4): 218-222.
[11]	王心　尚丽新. 妊娠中期糖尿病患者血清胱抑素C 水平与胰岛素抵抗关系的临床研究[J]. 发育医学电子杂志, 2015, 3(1): 39-41,49.

[1]	Society of Neonatologist, Chinese Medical Doctor Association. Consensus recommendations on the prevention and early management of respiratory distress syndrome in preterm infants[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 129 -131 .
[2]	Professional Committee of Respiratory, Society of Neonatologist, Chinese Medical Doctor Association. Clinical application recommendations for heated humidified high flow nasal cannula[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 132 -135 .
[3]	YAN Jun, ZHU Xing-wang, SHI Yuan. Application progress of noninvasive ventilate technique for premature infants[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 136 -140 .
[4]	GU Min-fang, YANG Chuan-zhong. Progress of intrapartum resuscitation for premature infants[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 141 -145 .
[5]	LIU Shu-hua, SHEN Yue-bo, LIU Cui-qing, MA Li. The efficacy of pulmonary surfactant for pulmonary function in premature tension pneumothorax[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 146 -151 .
[6]	GAO Xiao-hui, MAO Jian. Clinical features of non-oliguric hyperkalemia in extremely low birth weight infants[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 152 -158 .
[7]	XIA Yao-fang, YANG Juan , TIAN Bao-li, et al. Value of amplitude-integrated electroencephalography in monitoring acute period of neonatal bilirubin encephalopathy and prognostic assessment[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 159 -163 .
[8]	WANG Li-rong, SUN Xiao-yan, ZHU Ruo-xin, et al. Epidemiological investigation and analysis of women aged 40-55 years old with osteoporosis in Gansu province[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 164 -167 .
[9]	CHEN Ru-yue, SHEN Yun-yan, CHEN Qing , et al. Five cases about Henoch-Schönlein purpura complicated with central nervous system injury in children and literatures review[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 168 -171 .
[10]	ZHANG Ai-run, FANG Xiao-yi. Lung function testing of bronchopulmonary dysplasia for infants and children[J]. Journal of Developmental Medicine(Electronic Version), 2017, 5(3): 172 -176 .

Viewed

Full text

Abstract

Cited

Shared

Discussed