Histological observation of goblet cell and mucoprotein 2 expression in mucosa of septumarisen from type Ⅰ intestinal atresia
Ye Mao, He Feng, Song Yanbiao, et al
(1. Department ofGeneral Neonatal Surgery, Children's Hospital, Capital Institute of Pediatrics, Beijing 100020, China;2. Department of Biochemistry and Immunology, Capital Institute of Pediatrics, Beijing 100020, China;3. Department of Central Laboratory, the Second Hospital of Hebei Medical University, Hebei, Shijiazhuang050000, China; 4. Department of Surgery, Heilongjiang Hospital, Beijing Children’s Hospital, CapitalMedical University, Heilongjiang, Harbin 150010, China; 5. Department of Surgery, Changchun Children’sHospital, Jilin, Changchun 130061, China; 6. Department of Pathology, Heilongjiang Hospital, BeijingChildren’s Hospital, Capital Medical University, Heilongjiang, Harbin 150010, China; 7. Department ofGeneral Neonatal Surgery, Children's Hospital, Capital Institute of Pediatrics, Department of Physiology,Capital Institute of Pediatrics, Beijing 100020, China)
Abstract: 【Abstract】 Objective To observe the expression of goblet cell and mucoprotein 2 (MUC2) in mucosa of septum arisen from typeⅠintestinal atresia. Method Totally 9 septa located in intestinal wall with distanceless than 15 cm from Treitz ligament (septum group) were collected. The age of the patients' operation were1-3 days after birth. The normal intestinal wall tissues were harvested and set as the control during resectionand anastomosis in the same patient (control group), followed by serial tissue sectioning and followinghematoxylin-eosin (HE) staining, periodic acid-Scheff (PAS) staining, as well immunohistochemistry stainingfor histological observation. The semi-quantitative calculation was conducted for statistical comparison.Statistical method performed by independent sample t test. Result HE staining showed mucous of septumarisen from typeⅠintestinal atresia presented relatively normal histological structure with less villi andmalalignment. Submucosa muscular layer was irregular and proliferating collagen in subcutaneous layer. Theresidual goblet cells were less in septum group (36.6±2.6) compared with control group (72.5±3.7), withthe difference was significant (t=11.235, P=0.001). PAS staining indicated that glycoprotein in goblet cell wasdenser and immunohistochemistry staining showed MUC2 was less-expressed in septum group (0.085 0±0.005 4) compared with control group (0.115 6±0.014 4), with the difference was significant (t=18.652,P=0.014). Conclusion Compared with the normal intestinal wall, the septum arisen from typeⅠintestinalatresia has disorganized microscopic structure and relatively less villi, and present imperfect developmentalfeature, suggesting weaker defensive function induced by goblet cell in contrast to the normal intestinal wall.
叶茂 何峰 宋岩彪 高鹏 崔钊 王欢 刘雪来. 空肠Ⅰ型闭锁隔膜组织黏膜层杯状细胞和黏蛋白2 表达的组织学观察[J]. 发育医学电子杂志, 2024, 12(3): 199-203.
Ye Mao, He Feng, Song Yanbiao, et al. Histological observation of goblet cell and mucoprotein 2 expression in mucosa of septumarisen from type Ⅰ intestinal atresia. Journal of Developmental Medicine(Electronic Version), 2024, 12(3): 199-203.
2024, Vol. 12 
