Abstract: 【Abstract】 Objective To evaluate the interventional effect of neonatal umbilical vein catheterization checklist on reducing the incidence of catheter-related blood stream infection (CRBSI). Methods From March 1, 2014 to March 1, 2019, among the children admitted to the neonatal department of the First Hospital of Handan City, children with umbilical vein catheterization were included in the study, and they were randomly divided into the observation group (208 cases) and the control group (210 cases). Umbilical vein catheterization was completed within 24 hours after admission, and unified indications for extubation were used. Refering to the operation checklist, we developed a checklist for umbilical vein catheterization for the observation group, and recorded the indications and contraindications of the children, preparation and evaluation of the catheterization, surgical check during the catheterization, postoperative cathetermaintenance, and catheter removal. Control group were managed in the usual way. CRBSI infection rate means the number of CRBSI infection cases per 1 000 days of central venous catheter. Statistical analysis was performed t test, χ2 test, and binomial z test. Results There was no significant difference in the age of admission, gestational age at birth, gender, time of intubation, and source of the children between the observation group and the control group (P>0.05). In the observation group, none of the items were underprepared; in the control group, 56 cases were found to be missing items during surgery. Three patients in the observation group had no pre-operation evaluation records, while 102 patients in the control group did not record their evaluations. In the observation group, 23 patients with slow withdrawal of blood were recorded, and 12 patients were adjusted the length of the catheterization; the control group's medical records and the nursing form did not record any accidents during the operation. The total number of catheterization days in the observation group and the control group were 2 082 days and 2 103 days, the number of infections was 1 and 7 respectively, and the CRBSI infection rate was 0.48 and 3.32 persontimes per thousand catheter days respectively. The CRBSI infection rate in the observation group was lower than that in the control group(z = 2.10, P<0.05). Conclusions The use of checklists in the process of neonatal umbilical vein catheterization can help operators and post-maintenance staff to remain vigilant, eliminate potential safety hazards, prevent errors, continue to improve, ensure the safety of children, and effectively reduce the CRBSI infection rate.