Effects of maternal overnutrition during prepregnancy on organ development of offspring mice
1Department of Obstetrics and Gynecology,No. 210 Hospital of PLA,Dalian, 116021, China; 2Department of Obstetrics andGynecology, General Hospital of Beijing Military Command, 100700 Beijing, China; 3Department of Occupational and Environmental Health, Dalian Medical University,Dalian, 116044, China;4Department of Obstetrics and Gynecology, the affiliated Zhongshan Hospital of Dalian University, Dalian, 116001, China.
Abstract: Abstract Objective To investigate the effects of prepregnancy overnutrition on organ development of offspring mice. Methods A total of 20 female Kunming mice were randomly divided into two groups, control and study group, which were fed with lab chow and high fat and high sugar diet, respectively. After feeding 4 weeks, all female mice were mated with male mice. After delivery, all mothers reared their pups freely. During pregnancy and lactation, mothers of the two groups were continuously fed their corresponding diet. After weaning, all pups were fed with lab chow. Physical development of offspring was monitored from birth to postnatal 6 weeks. Organ coefficients of offspring major organs were determined and the morphology of them was observed through HE staining under microscope at postnatal 6 weeks. Results 1After feeding 4 weeks, the average maternal bodyweight and blood sugar of study group were significantly higher than those of control groupP0.05 . During neonatal and early adulthood period, the body weight of offspring of study group was significantly higher than that of control groupP0.05.2The organ coefficients of heart and liver were both significantly higher in offspring of study group than those of control groupP0.05. Pancreas/bodyweight in offspring of study group was significantly lower than that of control groupP0.05 .There was no significant difference in organ coefficients of spleen, kidney and brainP>0.05.3Under microscope,compared with offspring of control group, in offspring of study group, thickness of atrial and ventricular wall was thicker, liver cells presented slight edema, and boundary of white pulp??red pulp and marginal zone in spleen was obscure Conclusions: Maternal overnutrition during prepregnancy results in high birth weight and obesity during young adulthood, and morphological changes of some organs in offpsring. These morphological abnormities might be the anatomical basis of influence of maternal prepregnancy overnutrition on offspring long-life health. The profound mechanisms are worthy of further study.
关怀 冯和玺 白洁 张晶鑫 唐冬梅 尚丽新. 母鼠孕前营养过剩对子代小鼠成年早期脏器形态影响[J]. 发育医学电子杂志, 2014, 2(2): 93-97.
GUAN Huai, FENG He-xi, BAI Jie, et al. Effects of maternal overnutrition during prepregnancy on organ development of offspring mice. Journal of Developmental Medicine(Electronic Version), 2014, 2(2): 93-97.
2014, Vol. 2 
