含凝血酶敏感蛋白基序的解聚蛋白样金属蛋白酶 L1,白细胞介素 -6,羊膜细胞,早产 ," /> 含凝血酶敏感蛋白基序的解聚蛋白样金属蛋白酶 L1,白细胞介素 -6,羊膜细胞,早产 ,"/> ADAMTS L1,Interleukin-6,Amniotic cells,Preterm birth ,"/> <div> <span style="line-height:2;font-size:14px;">ADAMTS L1 通过白细胞介素 -6 介导羊膜</span><span style="line-height:2;font-size:14px;">细胞凋亡对早产的潜在影响</span> </div>
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发育医学电子杂志  2020, Vol. 8 Issue (4): 308-312    DOI: 10.3969/j.issn.2095-5340.2020.04.004
  围产医学   论著 |产科 |
ADAMTS L1 通过白细胞介素 -6 介导羊膜细胞凋亡对早产的潜在影响
李雯 李姗姗 申永梅 赵晓敏 常颖
天津市人类发育与生殖调控重点实验室 天津市中心妇产科医院 产前诊断中心,天津 300100
ADAMTS L1 may increase the preterm birth by regulating apoptosis of amniotic membrane cell through interleukin-6
Li Wen, Li Shanshan, Shen Yongmei, et al
Tianjin Key Laboratory of Human Development and Reproductive Regulation Prenatal Diagnosis Center, Tianjin Central Hospital of Obstetrics Gynecology, Tianjin 300100, China
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摘要 
【摘要】 目的  研究含凝血酶敏感蛋白基序的解聚蛋白样金属蛋白酶 L1(a disintegrin-like andmetalloproteinase with thrombospondin L1,ADAMTS L1)是否通过参与炎症调控过程影响羊膜细胞凋亡,从而成为早产发病的潜在影响因子。 方法 收集足月产、自发性早产的胎盘组织 20 例,将其分为足月分娩组和自发性早产组,每组 10 例。利用实时荧光定量 PCR 及蛋白质印迹法(Western blot 技术)检测其中 ADAMTS L1 和白细胞介素 -6(interleukin-6,IL-6)的 mRNA 及蛋白水平;体外利用 PMA 活化 THP-1 细胞系模拟炎症环境,并通过慢病毒敲降人羊膜细胞系 WISH 中 ADAMTS L1(敲低组),检测其中 ADAMTS L1 及 IL-6 的 mRNA 及蛋白水平,比较其与空白组的差别。 结果  相较于足月分娩组,自发性早组ADAMTS L1及IL-6的mRNA水平分别约增加了11、15倍,蛋白水平分别约增加了9、12 倍;同时,敲低 ADAMTS L1 减少 WISH 细胞凋亡,与空白组相比,敲低 ADAMTS L1 后,凋亡相关蛋白 caspase3 mRNA 水平降至空白组的 0.35,蛋白水平降至空白组的 0.30。 结论 本研究利用体外实验评估了炎症过程中巨噬细胞分泌的 IL-6 可以调节 ADAMTS L1,进而引起自发性早产。但是,需要进一步的研究来证实我们的发现。
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Abstract: 
【Abstract】 Objective To study whether a disintegrin-like and metalloproteinase with thrombospondin L1(ADAMTS L1) affects the apoptosis of amniotic membrane cells by participating in the process of interventional recombination, thereby becoming a potential factor of preterm birth. Methods First, we collected 20 placental tissues of full-term birth (FTB) and spontaneous premature birth (PTB),and divided them into FTB group(10 cases) and PTB group(10 cases). QRT-PCR and Western blot were used to detect the mRNA and protein levels of ADAMTS L1 and interleukin-6 (IL-6) . Then we activated THP-1 cell line by PMA to simulate the metabolic environment in vivo. ADAMTS L1 was knocked down by lentivirus in WISH cell line (knocked down group), mRNA and protein levels of ADAMTSL1 and IL-6 were detected, and the data of knocked down group were compared with control group.
Results The expression of ADAMTS L1 in preterm tissues was significantly increased, according to the full term birth women, the mRNA level of ADAMTSL1 and IL-6 increased 11 and 15 times respectively, the expression of ADAMTSL1 and IL-6 protein increased 9 and 12 times respectively. Knockdown of ADAMTS L1 reduced WISH cell apoptosis.Compared with the control group, after knocking down ADAMTS L1, the mRNA level of apoptosis-related protein caspase3 dropped to 0.35 of the control group, and the protein level dropped to 0.3 of the control group. Conclusion This study used in vitro experiments to evaluate that ADAMTS L1 may promote apoptosis of human IL-6 secreted by macrophages during inflflammation can regulate ADAMTS L1, which in turn can cause spontaneous preterm labor. However, further research is needed to confirm our findings.
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收稿日期:  2020-06-15                     发布日期:  2020-11-03     
基金资助: 
天津市重大疾病防治科技重大专项(18ZXDBSY00230);国家自然科学基金青年项目(81801456)
通讯作者:  常颖    E-mail:  changying4470@sina.com
引用本文:    
李雯 李姗姗 申永梅 赵晓敏 常颖.
ADAMTS L1 通过白细胞介素 -6 介导羊膜细胞凋亡对早产的潜在影响
[J]. 发育医学电子杂志, 2020, 8(4): 308-312.
Li Wen, Li Shanshan, Shen Yongmei, et al.
ADAMTS L1 may increase the preterm birth by regulating apoptosis of amniotic membrane cell through interleukin-6
. Journal of Developmental Medicine(Electronic Version), 2020, 8(4): 308-312.
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http://www.fyyxzz.com/CN/10.3969/j.issn.2095-5340.2020.04.004  或          http://www.fyyxzz.com/CN/Y2020/V8/I4/308
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