Correlation of TRL-2, 4 and MCP-1 with premature rupture of membrane and chorioamnionitis
Xu Qingyun, Jin Yingzi, Liu Mengnan, et al
(1.Department of Obstetrics, the Affiliated Hospital of Yanbian University, Jilin, Yanji 133000, China; 2. Department of Pediatrics, the Affiliated Hospital of Yanbian University, Jilin, Yanji 133000, China)
Abstract: 【Abstract】 Objective To explore clinical significance of TLR-2, TLR-4 and MCP-1 in the premature rupture of membrane and chorioamnionitis. Methods From September 2017 to January 2018, 52 patients with term premature rupture of membrane (TPROM group) , 46 patients with preterm premature rupture of membranes (PPROM group), and 40 term-birth women with intact fetal membrane (control group)were selected in the Department of Obstetrics, the Affiliated Hospital of YanbianUniversity. There were more than 5 neutrophil infiltrations on the chorionic and amniotic tissues underthe microscope, which was diagnosed as chorioamnionitis. The placenta and fetal membrane tissue were taken for immunohistochemical staining after birth, and the dyeing result was determined according to the semi-quantitative integration method. The umbilical cord blood of newborns (PPROM group) was taken to detect the MCP-1 content. Statistical analysis was performed by the rank sum test and the χ2 test. Results The positive rate of TLR-2 in the placental tissue [80.43% (37/46) vs 78.85% (41/52), respectively], and the positive rate of TLR-4 [86.96% (40/46) vs 82.69% (43/52)] in the PPROM group and the TPROM group were higher than those in the control group [positive rate of TLR-2 : 57.50% (23/40), positive rate of TLR-4: 62.50% (25/40)], which was statistical significance (P<0.05); however, there was no statistical significance between the PPROM group and the TPROM group (P>0.05). Among of 46 patients of PPROM group, 11 cases of chorioamnionitis and 35 cases of non-chorioamnionitis were found according to the pathological examination of placental membrane. Although the positive rate of TLR-2 [100.00% (11/11)] and positive rate of TLR-4 [90.91% (10/11)] in the placental tissue of the chorioamnion group were higher than those in the non-chorioamnion group [74.29% (26/35) vs 85.71% (30/35) respectively], but there were no statistical significance (P>0.05). The content of MCP-1 in umbilical cord blood of newborns in chorioamnion group [109.12 (74.72-222.12) ng/L] was higher than that in non-chorioamnionitis group [60.52 (48.52-135.12) ng/L], which was statistical significance (P<0.05). Conclusions The increased expression of TLR-2, 4 in placenta tissue may be related to the occurrence and development of PROM. The increase level of MCP-1 in neonatal umbilical cord blood can indicate the occurrence of chorioamnionitis.
许青云 金英子 刘梦南 刘春雨 金贞爱. Toll 样受体-2、4 及单核细胞趋化蛋白-1与胎膜早破和绒毛膜羊膜炎的相关性研究[J]. 发育医学电子杂志, 2020, 8(2): 129-134.
Xu Qingyun, Jin Yingzi, Liu Mengnan, et al. Correlation of TRL-2, 4 and MCP-1 with premature rupture of membrane and chorioamnionitis. Journal of Developmental Medicine(Electronic Version), 2020, 8(2): 129-134.
2020, Vol. 8 
